1. Chromatography is a physical method that is used to separate and analyse __________.

a) Complex mixtures 

b) Simple mixtures

c) Viscous mixtures

d) Metals

Answer: a

2. In chromatography, if a solute does not separate and remains on the start line _______.

a) The solute is insoluble in the solvent

b) The solute is soluble in the solvent

c) The solvent is insoluble in the solute

d) The solvent is soluble in the solute

Answer: a

3. In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?

a) Paper chromatography 

b) Liquid chromatography 

c) Column chromatography

d) Gas chromatography

Answer: c

4. The compound eluted last and retained more in Normal and Reversed phase are _____.

a) Polar and Non-polar

b) Non- polar and Polar

c) Both are in Polar

d) Both are in Non- polar

e) None of the above

Answer: a

5. Liquid chromatography can be performed in which of the following ways?

a) Only on plane surfaces 

b) Only in columns

c) Either in columns or on plane surfaces

d) Neither in columns nor on plane surfaces

Answer: c

6. Gas chromatography can be performed in which of the following ways?

a) Only in columns

b) Only on plane surfaces

c) Either in columns or on plane surfaces

d) Neither in columns nor on plane surfaces

Answer: a

7. In paper chromatography, the MOST soluble solute ______.

a) Does not move from the start point

b) Stays closest to the start point

c) Travels furthest away from the start point

d) Can be found in the middle of the chromatogram

Answer: c

8. In chromatography, the stationary phase can be ___________ supported on a solid.

a) Liquid or gas 

b) Solid or liquid

c) Solid only

d) Liquid only

Answer: b

9. In chromatography, which of the following can the mobile phase be made of?

a) Solid or liquid

b) Liquid or gas

c) Gas only

d) Liquid only

Answer: b

10. Which of the following types of chromatography involves the separation of substances in a mixture over a 0.2mm thick layer of an adsorbent?

a) Gas liquid

b) Column

c) Paper 

d) Thin layer

Answer: d

11. Which type of Chromatography is used to purify volatile substances?


b) TLC

c) GC

Answer: c

12. In Thin layer chromatography, the stationary phase is made of _________ and the mobile phase is made of _________.

a) Liquid, gas 

b) Liquid, liquid

c) Solid, liquid

d) Solid, gas

Answer: c

13. TLC is generally used as a qualitative analytical technique for ___________.

a) Determining the number of components in a mixture

b) Checking the purity of a compound

c) Following the course of a reaction

d) All of the above

e) None

Answer: d

14. In Gas-liquid phase chromatography, the stationary phase is composed of _________ and the mobile phase is made of _________

a) Solid, liquid

b) Liquid, liquid

c) Liquid, gas

d) Solid, gas

Answer: c

15. Chromatography separate the mixture of dyes on the basis of their _____________.

a) Density

b) Solubility

c) Gravity

d) Boiling point

Answer: d

16. Absorption in chromatography is a process of _______.

a) Partitioning

b) Attenuation

c) Bulk phenomenon

d) Surface property

e) None of the above

Answer: a

17. The form of end product of silica gel after polymerization used in HPLC packing material _____.

a) Hydrogel

b) Aquagel

c) Xerogel

d) Aerogel

e) None

Answer: c

18. Response time, is the time required for the detector to attain a percent of the final response signal (98%). Too slow a response time leads to ______.

a) Peak broadening

b) Peak tailing

c) Peak distortion

d) Peak doubling

e) None

Answer: c

19. Which of the following techniques is least useful for structural determination of small organics?

a) UV spectroscopy

b) NMR

c) GC-MS

d) FT-IR

e) Mass

Answer: a

20. Peak broadening is greatly contributed by ______.

a) Column fitting

b) Mobile phase flow rate/ viscosity

c) Stagnant mobile phase in column

d) Detector sensitiveness

e) Stationary phase nature

Answer: c

21. Activity coefficient of an ideal solution is _____.

a) >1.0

b) =0

c) =1.0

d) None

Answer: c

22. In GC, retention depends upon the _______.

a) Vapour pressure and polarity of the solute

b) Size and charge of the solute

c) The method of sample introduction

d) The type of detector used

e) Type of column used

Answer: a

23. Back diffusion occurs mostly in

a) SEC

b) Gas chromatography



e) TIC

Answer: b

24. In general the lamp used in variable wavelength detector for UV in HPLC _______.

a) Deuterium

b) Tungsten

c) Carbon arc

d) Xenon

e) Any one of the above

Answer: a

25. Prevention of back flow during pump reciprocation is done by ______.

a) Adapter

b) Back diffusion

c) Check valve

d) Degassing

e) Either of the above

Answer: c

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