HPLC and GC both techniques are used for separation and analysis of compounds. These are widely used in various fields such as pharmaceuticals, food and beverages, environmental analysis, and forensic science.
Mobile phase:
In HPLC, the mobile phase is a liquid, while in GC, it is a gas.
Stationary phase:
In HPLC, the stationary phase is a solid support, while in GC, it is a porous solid support.
Sample preparation:
In HPLC, the sample is dissolved in the mobile phase, while in GC, the sample is vaporized and injected into the GC column.
Separation mechanism:
HPLC separates compounds based on their interaction with the stationary phase and the mobile phase, while GC separates compounds based on their volatility and affinity to the stationary phase.
Applications:
HPLC is widely used in various fields such as pharmaceuticals, food and beverages, environmental analysis, and forensic science, while GC is commonly used in the analysis of volatile organic compounds in various fields such as environmental science, food and beverages, and forensic science.
Read also:
- Basic Knowledge on HPLC and GC
- Installation of GC Column
- Interview Questions and Answers on Chromatography
