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Overview of ISO 16140-3 | Method Verification

This guideline is especially beneficial for people working in Food Industry for improving the confidence in laboratory results. 

This guideline discuss on protocol for the verification of reference methods and validated alternative methods in a single laboratory. Which is applicable to the verification of methods used for the analysis (detection and/or quantification), confirmation and typing of microorganisms in:

— products intended for human consumption;

— products intended for animal feeding;

— environmental samples in the area of food and feed production, handling;

— samples from the primary production stage.

This document is, in particular, applicable to bacteria and fungi. Some clauses can be applicable to other (micro)organisms or their metabolites, to be determined on a case-by-case basis.


1. Choice of the method to be verified

2. Scope of validation of the method

3. Scope of the verification

4. Select (food) items

5. Protocol for verification

6. Analysis

7. Evaluation of results

The technical protocols for the verification of validated qualitative methods and validated quantitative methods are described in Clauses 5 and 6. The technical protocol for the verification of validated alternative confirmation and typing methods is described in Clause 7.

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